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plko lentiviral vector  (Addgene inc)


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    Structured Review

    Addgene inc plko lentiviral vector
    Plko Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lentiviral+vectors+plko+1/pLKO%2E1+puro+(Plasmid+%238453)/pm42863564-121-7-10
    Average 96 stars, based on 1591 article reviews
    plko lentiviral vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    shRNA:

    Article Title: Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into cultured HepG2 and Hep3B cells at the exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing SNRPFP1 transcript expression. ..

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene
    Article Snippet: The lentiviral vector pLV (Addgene, Cambridge, USA) was applied for the extopical expression of MT2P1 and its parental gene MT2A, in the exponential phase HepG2 and Hep3B cells (JIKE Biochemistry, Shanghai, China). .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into the HCC cells to deplete the expression of E2F7. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis.
    Article Snippet: .. Preparation of the plasmid and cell transfection The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: AMPKα loss promotes KRAS-mediated lung tumorigenesis.
    Article Snippet: AMP-activated protein kinase (AMPK) is a critical sensor of energy status that coordinates cell growth with energy balance.. In non-small cell lung cancer (NSCLC) the role of AMPKα is controversial and its contribution to lung carcinogenesis is not well-defined.. Furthermore, it remains largely unknown whether long non-coding RNAs (lncRNAs) are involved in the regulation of AMPK-mediated pathways.

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene.
    Article Snippet: The plasmid and cell transfection preparation The lentiviral vector pLV (Addgene, Cambridge, USA) was applied for the extopical expression of MT2P1 and its parental gene MT2A, in the exponential phase HepG2 and Hep3B cells (JIKE Biochemistry, Shanghai, China). .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into the HCC cells to deplete the expression of E2F7. ..

    Article Title: Alternative splicing-related long noncoding RNA ANRIL facilitates hepatocellular carcinoma by targeting the miR-199a-5p/SRSF1 axis and impacting Anillin.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: No. 82172900, No. 82372603, No. 81602544 Abstract Hepatocellular carcinoma (HCC) is characterized by aberrant alternative splicing (AS), which plays an important part in the pathological process of this disease.. However, available reports about genes and mechanisms involved in AS process are limited.. Our previous research has identified ANRIL as a long noncoding RNA related to the AS process of HCC.

    Transfection:

    Article Title: Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into cultured HepG2 and Hep3B cells at the exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing SNRPFP1 transcript expression. ..

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene
    Article Snippet: The lentiviral vector pLV (Addgene, Cambridge, USA) was applied for the extopical expression of MT2P1 and its parental gene MT2A, in the exponential phase HepG2 and Hep3B cells (JIKE Biochemistry, Shanghai, China). .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into the HCC cells to deplete the expression of E2F7. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis.
    Article Snippet: .. Preparation of the plasmid and cell transfection The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene.
    Article Snippet: The plasmid and cell transfection preparation The lentiviral vector pLV (Addgene, Cambridge, USA) was applied for the extopical expression of MT2P1 and its parental gene MT2A, in the exponential phase HepG2 and Hep3B cells (JIKE Biochemistry, Shanghai, China). .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into the HCC cells to deplete the expression of E2F7. ..

    Article Title: Alternative splicing-related long noncoding RNA ANRIL facilitates hepatocellular carcinoma by targeting the miR-199a-5p/SRSF1 axis and impacting Anillin.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: No. 82172900, No. 82372603, No. 81602544 Abstract Hepatocellular carcinoma (HCC) is characterized by aberrant alternative splicing (AS), which plays an important part in the pathological process of this disease.. However, available reports about genes and mechanisms involved in AS process are limited.. Our previous research has identified ANRIL as a long noncoding RNA related to the AS process of HCC.

    Cell Culture:

    Article Title: Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into cultured HepG2 and Hep3B cells at the exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing SNRPFP1 transcript expression. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis.
    Article Snippet: .. Preparation of the plasmid and cell transfection The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: Alternative splicing-related long noncoding RNA ANRIL facilitates hepatocellular carcinoma by targeting the miR-199a-5p/SRSF1 axis and impacting Anillin.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: No. 82172900, No. 82372603, No. 81602544 Abstract Hepatocellular carcinoma (HCC) is characterized by aberrant alternative splicing (AS), which plays an important part in the pathological process of this disease.. However, available reports about genes and mechanisms involved in AS process are limited.. Our previous research has identified ANRIL as a long noncoding RNA related to the AS process of HCC.

    Expressing:

    Article Title: Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into cultured HepG2 and Hep3B cells at the exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing SNRPFP1 transcript expression. ..

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene
    Article Snippet: The lentiviral vector pLV (Addgene, Cambridge, USA) was applied for the extopical expression of MT2P1 and its parental gene MT2A, in the exponential phase HepG2 and Hep3B cells (JIKE Biochemistry, Shanghai, China). .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into the HCC cells to deplete the expression of E2F7. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis.
    Article Snippet: .. Preparation of the plasmid and cell transfection The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis
    Article Snippet: .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Article Title: Suppression of pseudogene MT2P1 transcription induced by E2F7 inhibits hepatocellular carcinoma cell proliferation and facilitates apoptosis via preserving its parental gene.
    Article Snippet: The plasmid and cell transfection preparation The lentiviral vector pLV (Addgene, Cambridge, USA) was applied for the extopical expression of MT2P1 and its parental gene MT2A, in the exponential phase HepG2 and Hep3B cells (JIKE Biochemistry, Shanghai, China). .. The lentiviral vectors pLKO.1 (Addgene, Cambridge, USA) containing shRNA was transfected into the HCC cells to deplete the expression of E2F7. ..

    Article Title: Alternative splicing-related long noncoding RNA ANRIL facilitates hepatocellular carcinoma by targeting the miR-199a-5p/SRSF1 axis and impacting Anillin.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: No. 82172900, No. 82372603, No. 81602544 Abstract Hepatocellular carcinoma (HCC) is characterized by aberrant alternative splicing (AS), which plays an important part in the pathological process of this disease.. However, available reports about genes and mechanisms involved in AS process are limited.. Our previous research has identified ANRIL as a long noncoding RNA related to the AS process of HCC.

    Plasmid Preparation:

    Article Title: Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis.
    Article Snippet: .. Preparation of the plasmid and cell transfection The lentiviral vectors pLKO.1 (Addgene, Cambridge, MA, USA) containing shRNA were transfected into cultured HepG2 and Hep3B cells at exponential phase (JIKE Biochemistry, Shanghai, China) for suppressing the expression of UBE2MP1 transcript. ..

    Knockdown:

    Article Title: AMPKα loss promotes KRAS-mediated lung tumorigenesis.
    Article Snippet: AMP-activated protein kinase (AMPK) is a critical sensor of energy status that coordinates cell growth with energy balance.. In non-small cell lung cancer (NSCLC) the role of AMPKα is controversial and its contribution to lung carcinogenesis is not well-defined.. Furthermore, it remains largely unknown whether long non-coding RNAs (lncRNAs) are involved in the regulation of AMPK-mediated pathways.

    Control:

    Article Title: AMPKα loss promotes KRAS-mediated lung tumorigenesis.
    Article Snippet: AMP-activated protein kinase (AMPK) is a critical sensor of energy status that coordinates cell growth with energy balance.. In non-small cell lung cancer (NSCLC) the role of AMPKα is controversial and its contribution to lung carcinogenesis is not well-defined.. Furthermore, it remains largely unknown whether long non-coding RNAs (lncRNAs) are involved in the regulation of AMPK-mediated pathways.



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